Heartworms

Dirofilaria immitis, heartworm, or dog heartworm, is a  that is a type of, a small thread-like worm, that causes. It is spread from to host through the bites of es. The definitive host is the, but it can also infect s, , s, s, es, and other animals, such as s, , s, s and, under rare circumstances, s.

Dirofilaria immitis is commonly called "heartworm"; however, adults often reside in the pulmonary arterial system, as well as the heart, and a major effect on the health for the animal is a manifestation of damage to the lung vessels and tissues. In cases involving heavy worm burden, adult heartworms may migrate to the right heart and the. Heartworm infection may result in serious complications for the host, typically culminating in the host's death, most often as the result of secondary.

Distribution
Although at one time confined to the southern United States, heartworm has now spread to nearly all locations where its mosquito is found. In the southeast region of the United States, veterinary clinics saw an average of more than 100 cases of heartworm each in 2016. Transmission of the parasite occurs in all of the United States (cases have even been reported in ), and the warmer regions of. The highest infection rates are found within 150 miles of the coast from  to, and along the  and its major. It has also been found in, southern , , the, , Korea, and.

Course of infection
Heartworms go through several before they become adults infecting the pulmonary artery of the host animal. The worms require the mosquito as an intermediate host to complete their lifecycles. The rate of development in the mosquito is temperature-dependent, requiring about two weeks of temperature at or above 27 °C (80 °F). Below a threshold temperature of 14 °C (57 °F), development cannot occur, and the cycle is halted. As a result, transmission is limited to warm weather, and duration of the transmission season varies geographically. The period between the initial infection when the dog is bitten by a mosquito and the maturation of the worms into adults living in the pulmonary arteries takes six to seven months in dogs and is known as the "prepatent period".

The first larval stage (L1) and second larval stage (L2) of heartworm development occurs within the body of a mosquito. Once the larvae develop into the infective third larval stage (L3), the mosquito locates and bites a host, depositing the larvae under the skin at the site of the bite. After a week or two of further growth, they molt into the fourth larval stage (L4). Then, they migrate to the s of the and, and 45 to 60 days after infection, molt to the fifth stage (L5, immature adult). Between 75 and 120 days after infection, these immature heartworms then enter the bloodstream and are carried through the heart to reside in the pulmonary artery. Over the next three to four months, they increase greatly in size. The female adult worm is about 30 cm in length, and the male is about 23 cm, with a coiled tail. By seven months after infection, the adult worms have mated and the females begin giving birth to live young, called e.

The microfilariae circulate in the bloodstream for as long as two years, and are ingested by bloodsucking mosquitos, where development occurs and the cycle repeats.

Hosts
Hosts of Dirofilaria immitis include:


 * (rare)
 * s
 * (rare)
 * s
 * (rare)
 * s
 * (rare)
 * s
 * (rare)
 * s
 * (rare)
 * s
 * s
 * s

Reservoir hosts for D. immitis are coyotes and stray dogs.

Clinical signs of infection
Dogs show no indication of heartworm infection during the six-month prepatent period prior to the worms' maturation, and current diagnostic tests for the presence of e or s cannot detect prepatent infections. Rarely, migrating heartworm larvae get "lost" and end up in aberrant sites, such as the eye, brain, or an artery in the leg, which results in unusual symptoms such as blindness, s, and, but normally, until the larvae mature and congregate inside the heart, they produce no symptoms or signs of illness.

Many dogs show little or no sign of infection even after the worms become adults. These animals usually have only a light infection and live a fairly sedentary lifestyle. However, active dogs and those with heavier infections may show the classic signs of heartworm disease. Early signs include a cough, especially during or after exercise, and exercise intolerance. In the most advanced cases where many adult worms have built up in the heart without treatment, signs progress to severe weight loss,, coughing up blood, and finally, congestive heart failure.

Role of Wolbachia
 pipientis is an intracellular that is an  of D. immitis. All heartworms are thought to be infected with Wolbachia to some degree. The inflammation occurring at the die-off of adult heartworms or larvae is in part due to the release of Wolbachia bacteria or protein into the tissues. This may be particularly significant in cats, in which the disease seems to be more related to larval death than living adult heartworms. Treating heartworm-positive animals with an antibiotic such as to remove Wolbachia may prove to be beneficial, but further studies are necessary.

Diagnosis
Three methods can be used for the diagnosis:

Microfilarial detection was accomplished most commonly in the past by the microscopic identification of microfilariae on a, above the in a microhematocrit tube (or capillary tube), using the modified Knott test, or after millipore filtration. The accuracy of these tests, typically used for routine screening or diagnosis of heartworm infection, is improved by multiple testing. The modified Knott test and millipore filtration are more sensitive because they concentrate microfilariae, improving the chance of diagnosis. The direct smear technique allows examination of larval motion, helping in the distinction of D. immitis from Acanthocheilonema reconditum. This distinction is important because the presence of the latter parasite does not require expensive and potentially harmful therapy. The potential for a microfilaremic infection is 5 - 67%. The number of circulating microfilariae does not correlate with the number of adult heartworms, so is not an indicator of disease severity.

ing, in most practices, has supplanted or supplemented microfilarial detection. Combining the microfilaria and adult antigen test is most useful in dogs receiving or no preventive ( like  or  typically render the dog amicrofilaremic). Up to 1% of infected dogs are microfilaria-positive and antigen-negative. (,, rapid immunomigration techniques) to detect heartworm antigen in the host's blood are now regularly used. They can detect occult infections, or infections without the presence of circulating microfilariae. However, these tests are limited in that they only detect the antigens released from the sexually mature female worm's. Therefore, false-negative results may occur during the first five to eight months of infection when the worms are not yet sexually mature. The of these tests is close to 100%, and the sensitivity is more than 80%. A recent study demonstrated a sensitivity of only 64% for infections of only one female worm, but improved with increasing female worm burden (85%, 88%, and 89% for two, three, and four female worms, respectively). Specificity in this study was 97%. test results can be due to low worm counts, immature infections, and all-male infections.

 are used to evaluate the severity of the heartworm infection and develop a prognosis for the animal. Typically, the changes observed are enlargement of the main pulmonary artery, the right side of the heart, and the pulmonary arteries in the lobes of the lung. Inflammation of the lung tissue is also often observed.

Treatment
If an animal is diagnosed with heartworms, treatment may be indicated. Before the worms can be treated, however, the dog must be evaluated for heart, liver, and kidney function to evaluate the risks of treatment. Usually, the adult worms are killed with an -based compound. The currently approved drug in the US,, is marketed under the brand name Immiticide. It has a greater efficacy and fewer side effects than the previously used drug, sold as Caparsolate, which makes it a safer alternative for dogs with late-stage infections.

After treatment, the dog must rest (restricted exercise) for several weeks so as to give its body sufficient time to absorb the dead worms without ill effect. Otherwise, when the dog is under exertion, dead worms may break loose and travel to the lungs, potentially causing and death. According to the American Heartworm Society, use of in dogs infected with heartworms is no longer recommended due to a lack of evidence of clinical benefit and may be contraindicated. It had previously been recommended for its effects on platelet adhesion and reduction of vascular damage caused by the heartworms.

The course of treatment is not completed until several weeks later, when the microfilariae are dealt with in a separate course of treatment. Once heartworm tests are negative, the treatment is considered a success.

Surgical removal of the adult heartworms as a treatment also may be indicated, especially in advanced cases with substantial heart involvement.

Long-term monthly administration of year-round at three times the dose normally used for heartworm  eventually kills adult heartworms. However, this is not the treatment of choice for removal of adult heartworms for two reasons. First, this treatment is not as effective as melarsomine. More importantly, adult heartworms do not begin to die until 18 months of treatment have elapsed, which is not acceptable for dogs with high-volume infections and the worms cause irreversible damage to the heart and lungs during that time. Long-term treatment over a year with doxycycline daily and Heartgard Plus has been shown to be effective in early heartworm patients which are asymptomatic.

Prevention
Prevention of heartworm infection can be obtained through a number of veterinary drugs. The drugs approved for use in the US are ivermectin (sold under the brand names, , and several other generic versions), (Interceptor Flavor Tabs and ) and  (ProHeart) administered as pills or chewable tablets. Moxidectin is also available in both a six-month and 12-month, sustained-release injection, or, administered by veterinarians. The injectable form of moxidectin was taken off the market in the United States due to safety concerns in 2004, but the returned a newly formulated ProHeart 6 to the market in 2008. ProHeart 6 remains on the market in many other countries, including Canada and Japan. Its sister product,, is used extensively in Australia and Asia as a 12-month injectable preventive. It was approved for use in the United States by the in July 2019. Topical treatments are available, as well. Advantage Multi (imidacloprid + moxidectin) Topical Solution, uses moxidectin for control and prevention of s, s, heartworms, and s, as well as to kill adult s.    is a topical preventive likewise administered monthly; it also controls fleas, s, and s.

Preventive drugs are highly effective, and when regularly administered, protect more than 99% of dogs and cats from heartworm. Most compromises in protection result from failure to properly administer the drugs during seasonal transmission periods. In regions where the temperature is consistently above 14 °C (57 °F) year-round, a continuous prevention schedule is recommended.

Due to resistant strains of heartworms, in which no macrocyclic lactone (heartworm prevention) can protect against, the American Heartworm Society recommends dogs be on a repellent and a heartworm preventive. The repellent, such as Vectra 3-D, keeps mosquitoes from feeding on the dog and transmitting the L3 stage worms. If a dog is bitten, the heartworm preventive takes over when administered. If a mosquito feeds on a heartworm positive dog on a repellent, they do not live long enough for the microfilaria they ingested to molt into the ineffective L3 larva. Vectra 3-D was tested using thousands of mosquitoes infected with the resistant heartworm strain JYD34. In the control group that was given only a placebo, every dog contracted heartworms. In the experimental group that was given only Vectra 3-D, two of eight dogs contracted heartworms and had an average of 1.5 adult worms each. In the experimental group given both heartworm prevention and Vectra 3-D, one dog was infected with L3 stage larvae that did not mature into adulthood due to the heartworm prevention. Using a repellant and a prevention is over 99% effective.

Ivermectin, even with lapses up to four months between doses, still provides 95% protection from adult worms. This period is called the reach-back effect. Since dogs are susceptible to heartworms, they should be tested annually before they start preventive treatment. Annual heartworm testing is highly recommended for pet owners who choose to use minimal dosing schedules. Testing your dog annually for heartworms and other internal parasites is part of a complete prevention health care program. If your dog is on a monthly prevention, they should also be a part of this program.

Heartworm prevention for cats is available as ivermectin (Heartgard for Cats), milbemycin (Interceptor), or the topical selamectin (Revolution for Cats) and Advantage Multi (imidacloprid + moxidectin) topical solution.

Cats
While dogs are a natural host for D. immitis, cats are atypical hosts. Because of this, differences between canine and feline heartworm diseases are significant. The majority of heartworm larvae do not survive in cats, so unlike in dogs, a typical infection in cats is two to five worms. The lifespan of heartworms is considerably shorter in cats, only two to three years, and most infections in cats do not have circulating microfilariae. Cats are also more likely to have aberrant migration of heartworm larvae, resulting in infections in the brain or body cavities.

The infection rate in cats is 1-5% of that in dogs in areas. Both indoor and outdoor cats are infected. The mosquito vector is known to enter homes.

Pathology
The vascular disease in cats that occurs when the L5 larvae invade the is more severe than in dogs. A syndrome related to this inflammatory reaction has been identified in cats: heartworm-associated respiratory disease, which can occur three to four months after the initial infection, and is caused by the presence of the L5 larvae in the vessels. The subsequent inflammation of the pulmonary vasculature and lungs can be easily misdiagnosed as or allergic bronchitis.

Obstruction of pulmonary arteries due to from dying worms is more likely to be fatal in cats than dogs because of less  and fewer vessels.

Signs and symptoms
Acute heartworm disease in cats can result in, vomiting, diarrhea, fainting, and sudden death. Chronic infection can cause loss of appetite, weight loss, lethargy, exercise intolerance, coughing, and difficulty breathing. The signs of heartworm-associated respiratory disease can persist even after complete elimination of the heartworm infection. Normally, a cat’s immune response is strong enough to clear a heartworm infection; however, the immune response itself can cause the same signs associated with heartworm disease. If the infection is resolved, a cat can still have respiratory damage.

Diagnosis
Diagnosis of heartworm infection in cats is problematic. Like in dogs, a positive test for heartworm antigen is a very strong indication of infection. However, the likelihood of a positive antigen test depends on the number of adult female worms present. If only male worms are present, the test will be negative. Even with female worms, an antigen test usually only becomes positive seven to eight months after infection. Therefore, a cat may have significant clinical signs long before the development of a positive test. Heartworm-associated respiratory disease can be found in cats that never develop adult heartworms and therefore never have a positive antigen test.

An antibody test is also available for feline heartworm infection. It will be positive in the event of exposure to D. immitis, so a cat that has successfully eliminated an infection may still be positive for up to three months. The antibody test is more sensitive than the antigen test, but it does not provide direct evidence of adult infection. It can, however, be considered specific for diagnosing previous larval infections, and therefore fairly specific for heartworm-associated respiratory disease.

of the chest of a heartworm-infected cat may show an increased width of the pulmonary arteries and focal or diffuse opacities in the lungs. is a fairly sensitive test in cats. Adult heartworms appear as double-lined hyperechoic structures within the heart or pulmonary arteries.

Treatment and prevention
Arsenic compounds have been used for heartworm adulticide treatment in cats, as well as dogs, but seem more likely to cause pulmonary reactions. A significant number of cats develop s a few days after treatment. The effects of melarsomine are poorly studied in cats. Due to a lack of studies showing a clear benefit of treatment and the short lifespan of heartworms in cats, adulticide therapy is not recommended, and no drugs are approved in the US for use in cats.

Treatment typically consists of putting the cat on a monthly heartworm preventive and a short-term. Surgery has also been used successfully to remove adult worms. Ivermectin, milbemycin, and selamectin are approved for use in cats in the US. The prognosis for feline heartworm disease is guarded.

Human health considerations
Dirofiliaria are important medical parasites, but diagnosis is unusual and is often only made after an infected person happens to have a chest X-ray following granuloma formation in the lung; the nodule may be large enough to resemble on the X-ray and require a  for a pathologic assessment. This may well be the most significant medical consequence of human infection by the dog heartworm. People get this parasite by the bite of an infected mosquito, which is the same way dogs get infected.

D. immitis is one of many species that can cause infection in dogs and humans. It was thought to infect the human eye, with most cases reported from the southeastern United States. However, these cases are now thought to be caused by a closely related parasite of s, . Several hundred cases of infections in humans have been reported in Europe, but these are almost always caused by another closely related parasite, , rather than the dog heartworm. However, a few cases are proven D. immitis infections. Although there have been a few proven cases, humans mostly never get infected with heartworms due to the larvae never fully maturing. When the heartworm microfilariae are on their way through the skin, they often die since heartworms cannot survive in a human host, even if they make it into the bloodstream. When the heartworms die, the immune system in the human body reacts to their tissue with inflammation as it tries to destroy the heartworms. When this happens, the condition is called pulmonary dirofilariasis. Heartworms in humans is not a serious problem unless they are causing pain, discomfort, and other noticeable symptoms.